One of the most requested features for Serial Cloner was the ability to handle Features/Annotations, and this enhancement has now been fully implemented. Users can annotate any sequence portion, or the automatic scan feature can be used to detect common features. Serial Cloner is compatible with DNA Strider files, as well as the import and export of files in FASTA format. The new version allows for ApE and VectorNTI files to be read with their features, as well as pDRAW32 sequences. Genbank/EMBL files can be imported or read from the internet via the included web interface. The software provides powerful graphical display tools to facilitate analysis and construction processes in an intuitive way. #SERIAL BASICS FREE FR SERIAL CLONER SOFTWARE# All the tools needed for analyzing and manipulating sequences are contained in a single all-in-one-window concept, with key functions easily accessible via the compact interface. Serial Cloner also allows users to build text restriction maps, quickly formatting them to add multi-frame translation, or showing single cutters, for example. The graphic map of Serial Cloner provides a truly graphic interface, where users can easily select and extract a feature, fragment, or show single, double or multiple cutters all in the same window. Using Serial Cloner, users can set up new sub-cloning projects and prepare electronic versions of constructs. It provides classical restriction maps, both graphic and text-based, as well as site usage windows. Users can also set up PCR-based amplification quickly or prepare synthetic adaptors. shRNA constructions based on pre-defined scaffolds are automated, while assembling fragments obtained by PCR, adaptor/shRNA synthesis, or graphically selecting fragments between restriction sites is simplified with just a few clicks. Gateway cloning for both BP and LR reactions is easier with Serial Cloner, and it also provides an interface to align two sequences using a local algorithm or the BLAST2Seq NCBI server and extract a consensus a virtual cutter, a web browser with instant import of NCBI/EMBL entries, and a silent restriction map generator. #SERIAL BASICS FREE FR SERIAL CLONER SERIAL# #SERIAL BASICS FREE FR SERIAL CLONER SOFTWARE#.#SERIAL BASICS FREE FR SERIAL CLONER SERIAL#.In conclusion, Serial Cloner is a very user-friendly and powerful Molecular biology tool. Fragments obtained by PCR, shRNA synthesis, can be easily assembled by selecting between the restriction sites.We can also ligate fragments using the ligate button.Also, to create new PCR based fragments or synthetic adaptors. We can use either the graphics window or the text-based site usage windows, which makes it very easy to quickly extract a sub-sequence in a selection or in any restriction sites.There is a web browser tool within the software to allow direct import of NCBI and EMBL.Sequence features are visible on the sequence map.They can be automatically found by using the scan feature. Features can be manually entered or imported.The fully graphical map makes it easy to extract and select a fragment or even show single, double, or multiple cutters in a single window.All the tools required to analyze the sequence and modify if any changes needed are available in a single window making it very easy to use.The software comes with a high-quality graphics display and has a very user-friendly interface to the front end, which makes the interface very intuitive. They are processed both in sequence and as a graphic map. Since version 2.0 of the software, it also handles Annotations and features. It can read and write files in several formats, making it portable across multiple users who use different Molecular Biology tools. It is available free for download and can be used across multiple Operating Systems, including Windows, Mac, and Linux. Serial Cloner is a Molecular Biology Tool which is a front end to help with DNA Cloning, Sequence Analysis, and Visualization.
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